Shigatoxigenic Escherichia coli strains are major food-borne pathogens that are trans-
mitted through contaminated meat and meat products. Apart from cattle, sheep and
goat are important natural reservoirs. Faecal contamination during slaughter and fur-
ther processing along with poor hygienic practices accounts for the presence of STEC
in raw meat. A total of 200 samples (50 each of mutton and chicken and 50 samples
each of mutton and chicken swabs) collected from various sources were subjected to
PCR analysis for the presence of Shigatoxigenic E. coli using primers specifc to STEC
virulent genes, shiga toxin (stx1 and stx2) and enterohaemolysin (hlyA) with amplif-
cation size of 614bp, 779bp and 361bp respectively. Out of 200 samples, 58 showed
presence of STEC (20 mutton, 24 mutton swabs, 7 chicken and 7 chicken swabs out
of 50 samples each). Of the 58 STEC positive samples, 42 (72.4%) showed presence
of stx1, 16 (27.5%) showed stx2, 21 (36.2%) showed hlyA gene and 8 (13.7%) showed
both stx1 and stx2. Among 58 STEC positive isolates, 8.6% (1 mutton, 2 mutton swab
and 2 chicken swabs) isolates were possessing all the 3 virulent genes. The sensitivity
of PCR method for STEC was 1.7cfu. Enrichment with mTSB broth containing novo-
biocin gave good results compared to mEC broth with novobiocin. It is advisable
to use more rapid, sensitive and specifc PCR method to detect virulent STEC from
diferent food sources.
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Título : Archives of Clinical Microbiology: PCR analysis of mutton and chicken
samples for the presence of Shiga
Toxigenic E. coli
EAN : cdlcm19898429
Editorial : Fundación de Neurociencias
Fecha de publicación
: 3/4/12
Formato : PDF
Tamaño del archivo : 1.14 mb
Protección : Adobe DRM
El libro electrónico Archives of Clinical Microbiology: PCR analysis of mutton and chicken
samples for the presence of Shiga
Toxigenic E. coli está en formato PDF
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